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superscript iii first-strand synthesis system rt-pcr cdna synthesis kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript iii first-strand synthesis system rt-pcr cdna synthesis kit
    Superscript Iii First Strand Synthesis System Rt Pcr Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+synthesis+kit+superscript+iii+first-strand+synthesis+system+for+rt-pcr/pmc11443445-115-15-25
    Average 90 stars, based on 1 article reviews
    superscript iii first-strand synthesis system rt-pcr cdna synthesis kit - by Bioz Stars, 2026-11
    90/100 stars

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    Isolation:

    Article Title: Serial Profiling of Circulating Tumor DNA Identifies Dynamic Evolution of Clinically Actionable Genomic Alterations in High-Risk Neuroblastoma
    Article Snippet: TaqMan gene expression assays were used to quantitate ERRFI1 (Hs01086257_g1) and HPRT1 (Hs02800695_m1). qPCR analysis was performed on an Applied Biosystems 7900HT Sequence Detection System using standard cycling conditions, and relative expression values were calculated using the delta-delta Ct method.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Serial Profiling of Circulating Tumor DNA Identifies Dynamic Evolution of Clinically Actionable Genomic Alterations in High-Risk Neuroblastoma
    Article Snippet: TaqMan gene expression assays were used to quantitate ERRFI1 (Hs01086257_g1) and HPRT1 (Hs02800695_m1). qPCR analysis was performed on an Applied Biosystems 7900HT Sequence Detection System using standard cycling conditions, and relative expression values were calculated using the delta-delta Ct method.



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    MKP-2 deletion negatively affects Csf2 and Mmd gene expression in bone marrow-derived macrophages. Cells from day 3 of culture, were rendered quiescent in macrophage colony stimulating factor (M-CSF) free medium and then stimulated with recombinant M-CSF (10 ng/ml) for the indicated periods of time. Control cells (0) were left untreated. Total RNA was prepared from the cells. After reverse transcription, quantitative PCR analysis was performed <t>on</t> <t>cDNA</t> while using primers designed to detect Csf2 ( A ) and Mmd ( B ). Expression levels of Mmd and Csf-2 mRNA transcripts were normalized to the reference gene QARS using the delta-delta Ct method . Error bars represent the mean ± SEM from <t>three</t> individual experiments. * p < 0.05, one-tailed unpaired t test comparing MKP-2 -/- to MKP-2 +/+ macrophages.
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    MKP-2 deletion negatively affects Csf2 and Mmd gene expression in bone marrow-derived macrophages. Cells from day 3 of culture, were rendered quiescent in macrophage colony stimulating factor (M-CSF) free medium and then stimulated with recombinant M-CSF (10 ng/ml) for the indicated periods of time. Control cells (0) were left untreated. Total RNA was prepared from the cells. After reverse transcription, quantitative PCR analysis was performed on cDNA while using primers designed to detect Csf2 ( A ) and Mmd ( B ). Expression levels of Mmd and Csf-2 mRNA transcripts were normalized to the reference gene QARS using the delta-delta Ct method . Error bars represent the mean ± SEM from three individual experiments. * p < 0.05, one-tailed unpaired t test comparing MKP-2 -/- to MKP-2 +/+ macrophages.

    Journal: International Journal of Molecular Sciences

    Article Title: Whole Genome Microarray Analysis of DUSP4-Deletion Reveals A Novel Role for MAP Kinase Phosphatase-2 (MKP-2) in Macrophage Gene Expression and Function

    doi: 10.3390/ijms20143434

    Figure Lengend Snippet: MKP-2 deletion negatively affects Csf2 and Mmd gene expression in bone marrow-derived macrophages. Cells from day 3 of culture, were rendered quiescent in macrophage colony stimulating factor (M-CSF) free medium and then stimulated with recombinant M-CSF (10 ng/ml) for the indicated periods of time. Control cells (0) were left untreated. Total RNA was prepared from the cells. After reverse transcription, quantitative PCR analysis was performed on cDNA while using primers designed to detect Csf2 ( A ) and Mmd ( B ). Expression levels of Mmd and Csf-2 mRNA transcripts were normalized to the reference gene QARS using the delta-delta Ct method . Error bars represent the mean ± SEM from three individual experiments. * p < 0.05, one-tailed unpaired t test comparing MKP-2 -/- to MKP-2 +/+ macrophages.

    Article Snippet: SuperScript ® III First-Strand Kit (cDNA synthesis system for RT-PCR), oligonucleotide PCR Primers, SYBR ® Select real-time PCR Master Mix were purchased from Applied Biosystems, Life Technologies (Paisley, UK).

    Techniques: Expressing, Derivative Assay, Recombinant, Real-time Polymerase Chain Reaction, One-tailed Test